Identification of transgelin-2 as a biomarker of colorectal cancer by laser capture microdissection and quantitative proteome analysis
نویسندگان
چکیده
منابع مشابه
Laser capture microdissection and colorectal cancer proteomics.
The ability to define protein profiles of normal and diseased cells is important in understanding cell function. Laser capture microdissection permits the isolation of specific cell types for subsequent molecular analysis. In this study we have established conditions for obtaining proteomic information from laser capture microdissected colorectal cancer cells. Laser capture microdissection was ...
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The search to date for accurate protein biomarkers in acute ischemic stroke has taken into consideration the stage and/or the size of infarction, but has not accounted for the site of stroke. In the present study, multiple reaction monitoring using labeled reference peptide (LRP) following laser capture microdissection (LCM) is used to identify site-specific protein biomarker candidates. In mid...
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The acquisition of homogeneous or pure cell populations for cell biologic and molecular analyses has been a difficult challenge for many decades. A variety of approaches have been tried, including macroscopic dissection of tissues from frozen tissue blocks to increase the population of specific cell types (1,2). Some investigators have used irradiation of manually ink-stained sections to destro...
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ژورنال
عنوان ژورنال: Cancer Science
سال: 2010
ISSN: 1347-9032,1349-7006
DOI: 10.1111/j.1349-7006.2009.01424.x